Species Strain n ED200 ELGV, µg/kg (95% confidence interval) Relative Potency
Guinea pig Hartley 25 1.6 (1.0-2.3) 1
Hamster Golden Syrian 25 2232.0 (769.0-3033.0) 1,395
Mouse A/J 25 26.0 (16.6-41.2) 16
BALB/c 48 359.0 (185.0-641.0) 224
ICR 30 71.9 (29.8-155.0) 45
Rat Brown-Norway 20 24.1 (17.0-32.0) 15
Fischer 344 20 24.2 (12.1-38.6) 15
Lewis 20 28.8 (22.6-35.6) 18
Sprague-Dawley 20 17.2 (9.0-26.4) 11
Table 1: Table showing relative ED200 ELGV of methacholine producing pulmonary gas trapping increases in the guinea pig, hamster, mouse, and rat (from Stengel et al. [23]). Estimated inhaled dose of methacholine was calculated based on nebulizer solution concentration, usable nebulizer output, inhalation time, chambre airflow, minute volume, and animal body weight.