Time Flow A% B%
0.00 0.2 90 10
2.00 0.2 72 28
4.00 0.2 50 50
6.00 0.2 50 50
8.00 0.2 30 70
10.00 0.2 0 100
10.20 0.2 90 10
Table 1: Mobile phase gradient.