Figure 2: The expression of TfR1, DMT1 (+IRE), DMT1 (-IRE) and FPN1 protein was detected in rat brain after Glu treatment with western blot. A E show the protein expression in SN, striatum, Hippo, CC and cerebellum respectively. F shows the analysis of immunostained bands and its statistics. The expression of DMT1 (+IRE) protein was normalized to β-actin and all represented data was the relative values to ACSF group (0 μg Glu). All Data were expressed as means ± SD. * p<0.05 vs. 0 μg Glu, **p<0.01 vs. 0 μg Glu (n=6).