Figure 5: The expressions of iron transport proteins were detected in PC12 cells after Glu treatment with western blot analysis. After PC12 cells were incubated with Glu (0, 1, 10, 100, 1000 μM) for 16 h as described in Materials and Methods, the expression of DMT1 (+IRE) (A) and DMT1 (-IRE) (B) protein was increased at concentrations of 1 μM, 10 μM and 100 μM Glu, compared with that of control (0 μM Glu). While there were no changes of TfR1 (C) and FPN1 (D) expression in PC12 cells incubated with Glu. All histograms represent the relative values to the control group’s (0μM Glu) after normalization by β-actin. Data were expressed as means ± SD (relative to that of the 0 μM Glu group). * P<0.05 vs. 0 μM Glu group; **P<0.01 vs. 0 μM Glu group (n=6).