Figure 6: The distribution of NF-κB was translocated from cytoplasm into nucleus in PC12 cells after Glu treatment with immunocytochemistry. PC12 cells were treated with 100 μM Glu for 15 min, 30 min, 1 h, 2 h and 4 h. The left showed the cell nuclei staining with DAPI (blue), the middle showed the immunostaning of NF-κB (green), and the right showed the merged images. The upper three photomicrographs showed the staining of the control group, and the lowered parts showed the staining of Glu-treated group as indicated. The staining of NF-κB was observed obviously in the nucleus after Glu treatment for 30min. Scale bar: 5 μm (n=4).