Figure 3: Expression of NRSF target genes during traumatic stress. SD rats were divided into three groups: control, day 1, day 3 and day 7 (1, 3 and 7 days after trauma; n=5). The prefrontal cortex was collected and homogenized, and expressions of NRSF target genes including CCR5, CCR3, CD14, gp130 and IL-8 were detected by real-time PCR (A). Neurons and astrocytes were separated from prefrontal cortex and cultured for the indicated times; afterwards, cells were exposed to IL-1β/IL-1ra or IGF-1/IGF-1Ab. The expressions of CCR5, CCR3, CD14, gp130 and IL-8 in neurons (B), astrocytes (C), and coculture of neurons and astrocytes (D) was carried out by real-time PCR. Data were calculated as percentage of control; each value represents the mean ± S.E.M. of three independent experiments. *p<0.05 vs. control.