Figure 6: Nucleus GR expression during acute and chronic stresses. SD rats were underwent traumatic stress, or were subjected to predator scent exposure following trauma (trauma+PSS) for 14 days, and CCR5 was over-expressed by adeno-virus, the nucleus fraction was separated from prefrontal cortex, and GR expression was determined by western blot analysis (A). SD rats underwent traumatic stress, were subjected to predator scent exposure following trauma (trauma+PSS) for 14 days. (A) Prefrontal cortex was collected and homogenized, connection of NRSF and GR was determined by immunoprecipitation, in which anti-GR was used as the immunoprecipitated antibody, and anti-NRSF as the immunoblot antibody (B). In a co-culture of neurons and astrocytes: (C) after exposure to IL-1β/IL-1ra or IGF-1/IGF-1Ab, the interaction between NRSF and GR was determined by immunoprecipitation, in which anti-GR was used as the immunoprecipitated antibody, and anti-NRSF as the immunoblot antibody; (D) after transfection of Mock or NRSF, the dose response of GR activity to DEX was measured by luciferase assay; (E) DEX-induced nuclear GR expression was determined by western blot analysis. Data were calculated as percentage of control; each value represents the mean ± SEM of three independent experiments. *p<0.05 vs. control or Mock.