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Figure 1: UV chromatograms showing metabolites formed after 120 minute incubation of human liver microsomes in the presence of A) 10 μM BAT without NADPH or B) 10 μM BAT with NADPH C) 10 μM NAB without NADPH or D) 10 μM N-acetyl BAT with NADPH. E) 10 μM NAB + 0.1 mM BNPP without NADPH. F) 10 μM NAB + 0.1 mM. BNPP with NADPH. BNPP inhibits conversion of NAB to BAT in active HLM. |