| Fractions |
Bio-Chemical Assay |
|
|
Concentration (mg/ml) |
|
|
| |
|
0.05/0.2 |
0.10/0.4 |
0.15/0.6 |
0.20/0.8 |
0.25/1.0 |
0.30/1.2 |
| 0% ethanol |
A |
0.16 |
3.24 |
7.59 |
6.01 |
11.88 |
15.43 |
| B |
0.43 |
0.68 |
1.92 |
1.28 |
2.6 |
3.77 |
| C |
0.11 |
0.19 |
0.36 |
0.85 |
1.03 |
1.21 |
| D |
2.53 |
6.47 |
12.58 |
10.01 |
16.01 |
20.22 |
| 20% ethanol |
A |
0.12 |
2.18 |
5.37 |
2.61 |
6.77 |
8.4 |
| B |
0.18 |
0.32 |
0.69 |
1.34 |
2.18 |
4.82 |
| C |
0.1 |
0.39 |
0.33 |
0.46 |
0.89 |
1.06 |
| D |
2.68 |
5.37 |
7.98 |
9.56 |
21.32 |
26.49 |
| 40% ethanol |
A |
0.18 |
3.73 |
4.51 |
4.98 |
9.85 |
15.61 |
| B |
0.1 |
0.32 |
0.38 |
0.08 |
1.06 |
3.49 |
| C |
0.28 |
0.25 |
0.32 |
0.37 |
1.06 |
1.59 |
| D |
1.65 |
5.29 |
9 |
9.82 |
22.91 |
25.66 |
| 80% ethanol |
A |
0.38 |
5.29 |
8.64 |
6.78 |
13.56 |
19.23 |
| B |
0.68 |
0.76 |
2.64 |
3.53 |
3.58 |
6.91 |
| C |
0.56 |
0.58 |
0.64 |
0.49 |
1.27 |
1.88 |
| D |
5.2 |
9.56 |
15.46 |
11.28 |
21.01 |
28.97 |
|
|
|
| Biochemical analysis of the four different fractions extracted from the leaves of Vallisneriaspiralis L. A- Phenols; B-Flavonoids; C-Tannins and D-Antioxidant activity. The concentrations 0.5mg/ml-3.0mg/ml are for phenols, Tannins and Antioxidant activity and 0.2mg/ml-1.2mg/ml is specific for Flavonoid assay. |
| Table 1: Biochemical Analysis. |
