Sl.No. Microorganisms Initial stage of mine spoil Top soil Time period after reclamation 6th year 12th year 18th year 24th year 1 Actinomycetes (CFU/g) 0 4.5×104 8.1×102 2.1×104 8.5×105 9.5×105 2 Fungi (CFU/g) 0.1×101 5×102 3.8×102 5.6×103 9×104 6.2×105 3 Total bacteria (CFU/g) 0.6×101 1.9×104 7.9×104 1×107 5×109 8.6×109 4 Rhizobium (CFU/g) 0 5×101 5.4×102 2.2× 103 5×105 5×105 5 Azotobacter (CFUg-1) 0 2×101 7×102 3.6×105 4.1×107 4.0×107 6 VAM/10 g 0 9 12 28 48 51

Table 3: Microbial properties of 24 year old restored mine site at Gumgaon.