Figure 3: Scatterplots of flowering time (days after germination) as a function of the altitude of the source population in the three treatments: (left) 22-C day temperature with no vernalisation, (middle) 22-C day temperature with a 5-C vernalisation treatment followed by a return to the 22-C day temperature, and (right) 15-C day temperature with a 5-C vernalisation treatment followed by a return to the 15-C day temperature. Points represent the mean value for each lineage for the first (blue) and second (gold) laboratory generations of ssp. kamchatica and the second laboratory generation of ssp. kawasakiana (red). Regression lines for the left and middle plots were derived from the same best-fit non-linear mixed-effects model based on data from individual plants, with population and lineage as random effects (Table 4). The data in the right plot were obtained in a different growth chamber and were analysed separately (Table 5). In the middle graph, the colours of the three lines correspond to those of data points. The red line for ssp. kawasakiana is drawn within the short altitude range of this subspecies. Where a single black line is shown (left and right graphs), the flowering time for different generations and subspecies were regressed together.