Independent variables Dependent variable
Formulation Insulin (mg.mL-1) Lipid Emulsion (%) Transferrin (mg.mL-1) Viability (%)
1 1 1 1 57
2 10 1 1 64
3 1 3 1 92
4 10 3 1 90
5 1 1 5 69
6 10 1 5 60
7 1 3 5 91
8 10 3 5 78
9 5 2 3 82
10 5 2 3 78
11 5 2 3 79
Control n.a. n.a. n.a. 95
Formulations 9 to 11 correspond to central points, n.a.: not added. Control: Keratinocyte SFM (KCM)
Table 1: 23 factorial design for insulin, transferrin and lipid emulsion supplements.