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Figure 1: Four-color staining immunophenotyping analyses of Sample I. The following rat antibody conjugates, coupled with fluorescein isothiocyanate (FITC), phycoerythrin (PE), CyChrome (PE-Cy5), or allophycocyanin (APC) were used: CD45-PE-Cy5 (clone HI30), CD34-FITC (clone 581), CD49d-PE (clone 9F10), CD105- FITC (clone 266), CD73-PE (clone AD2), CD90-FITC (clone 5E10), and conjugated 7AD-annexin (Becton Dickinson). The respective isotype matched control was PE MoAb. |