AT-MSC |
CD34- |
CD45- |
CD49d+ |
CD73+ |
CD90+ |
CD105+ |
ANNEXIN+ |
7-AAD+ |
I (a) |
99.64 |
99.43 |
84.07 |
99.58 |
99.67 |
98.55 |
4.41 |
4.98 |
II (b) |
99.64 |
99.83 |
96.38 |
99.84 |
99.95 |
99.41 |
6.48 |
8.43 |
III (c) |
98.81 |
99.99 |
94.09 |
99.12 |
99.52 |
99.84 |
13.69 |
12.98 |
IV (d) |
96.68 |
99.85 |
93.74 |
99.66 |
99.86 |
99.80 |
4.78 |
7.35 |
V (e) |
99.19 |
99.88 |
88.77 |
99.88 |
99.87 |
99.91 |
3.8 |
5.78 |
VI (f) |
97.04 |
99.91 |
81.24 |
99.45 |
99.86 |
99.20 |
9.6 |
4.83 |
AT-MSC: adipose-tissue mesenchymal stem cells; CD34: CD34 FITC (clone 581); CD45: CD45 PE-Cy5 (Clone HI30); CD49d: CD49d PE (clone 9F10); CD73: CD73
PE (clone AD2); CD90: CD90 FITC (clone 5E10); CD105: CD105 FITC (clone 266); 7-Aminoactinomycin D (7-AAD) - dead cells. Annexin V - Apoptosis as well as other
forms of cell death |