Figure 1: Isolation and retrieval of c-Kit positive CSCs from human right atrial appendages (RAA). A. RAA was minced into small pieces, B. enzymatically digestion tissue with collagenase solution, C. undigested debris filtered out, D. cells grown in vitro, E. sorting of c-kit +ve cells by magnetic beads, F. staining of c-kit protein expressed by human CSCs.