Figure 5: Up-regulation of a novel set of human embryonic neural-driving miRNAs upon neural induction by RA.
(A) Hierarchal clustering of differentially expressed miRNAs in neural-induced hESCs by RA, compared with pluripotent hESCs. Statistically significant p values and clustering analysis were provided by LC Sciences as part of miRNA microarray service.
(B) A group of neural-driving miRNAs displayed an expression pattern of up-regulation upon neural induction and down-regulation upon cardiac induction.
(C) A group of neural-driving miRNAs had an expression pattern of up-regulation upon neural induction but was not significantly affected upon cardiac induction.
(D) Log2 ratios of up-regulation of the human embryonic neural-driving miRNAs. The levels of up-regulation: *: 5-10 fold, **: 10-50 fold, and ***: 50-200 fold.