Figure 2: Stemness markers Oct3/4, Sox2 and Nanog are expressed in DPSCs. Immunostainings of intracellular stemness markers Oct3/4 (A), Sox2 (D) indicator of nuclei DAPI (B, E) and merged images of Oct3/4 and Dapi (C) and Sox2 and Dapi (F). Scale bar =200 μm. Gene expression of stemness markers were analyzed by qRT-PCR in different media, SF/XF-M, HS-M and FBS-M (G). Data represents the mean ± SD and are normalized to housekeeping gene RPLP0. (n = 4, *p < 0.05, **p< 0.01 ***p< 0.001).