Figure 5: Response of FBS-M, HS-M and SF/XF-M on DPSCs proliferation assessed by WST-1 proliferation assay at day 1, 4, 7, 11 and 14. Cell proliferation in FBS-M, HS-M increased significantly from day 7 to 14 in comparison to cells cultured in SF/XF-M (A). Comparative analysis of DPSCs proliferation in varying concentrations of HS serum 5%-20%.The significant increase in proliferation was observed in serum concentration 10%, 15%, 20% at day 7 and 14 in comparison to 5%HS-M (B). Cell proliferation in SF/XF-M+1%HS increased significantly from day 4 to 7 in comparison to SF/XF-M (C). The bars represent the mean ± SD (n = 4, asterisks indicate significant differences, *p < 0.05, **p< 0.01, ***p< 0.001). DPSCs: dental pulp stem cells; FBS-M: fetal bovine serum medium; HS-M: human serum medium; SF/XF-M: serumfree/xenofree medium.