Figure 7: Chondrogenic differentiation analysis for DPSCs cultured in chondrogenic differentiation media in comparison to cells cultured in FBS, HS and SF/XF media. Histological staining by Alcian blue stain of DPSCs micromasses showing chondrogenic differentiation with more proteoglycan content in FBS isolated cells (A) (Scale Bar=100 μm) and qRT-PCR analysis of chondrogenic differentiation related gene expression for SOX9 (B) and COL10A1 (C). Data represents the mean ± SD and are normalized to housekeeping gene RPLP0. (n = 4, *p < 0.05, **p< 0.01 ***p< 0.001). (Online version in colour).