Medium Abbreviation Basal medium Serum Xenofree Coating Supplementation Fetal Bovine Serum- Medium FBS-M DMEM/F -12 10% FBS No No 1% GlutaMAX, 1% a/a Human Serum- Medium HS-M DMEM/F -12 20% HS Yes No 1% GlutaMAX, 1% a/a Serum Free/ XenoFree- Medium (Stem Pro® MSC SFM XenoFree) SF/XF-M Stem Pro®MSC SFM xenofree No Yes CELLstart (Life Technologies) Stem Pro® MSC SFM Xeno Free supplement, 1% a/a

Table 1: Different culture media to test the growth of DPSCs. Dulbecco’s modified eagle medium (DMEM/F-12) containing fetal bovine serum (FBS-M), human serum (HS-M) and serumfree/xenofree (SF/XF-M).