Medium |
Basal Media |
Serum |
Supplementation |
Control (FBS-M, HS-M, SF/XF- M) |
DMEM/F-12, SF/XF-M |
10% FBS or 15% HS or no serum |
None |
Osteogenic Medium (OM; FBS-OM, HS-OM, SF/XF-OM) |
DMEM/F-12, SF/XF-M |
10% FBS or |
50 µM L- ascorbic acid (Sigma), 10 |
15% HS or no |
mM beta glycerophosphate (Sigma), |
serum |
100nM 1,25 hydroxy Vitamin D3 |
|
(VD; Sigma), 1% a/a, 1% GlutaMAX |
Adipogenic Medium (AM; FBS-AM, HS-AM, SF/XF-AM) |
DMEM/F-12, SF/XF-M |
10% FBS or 15% HS or no serum |
33 µM biotin (Sigma) 1µM |
dexamethasone (Sigma), 100 nM |
insulin (life technologies), 17 µM |
pantothenate (Fluka), 1% GlutaMAX, |
and 1% a/a. Upon seeding of cells, |
250 µM of isobutylmethylxanthine |
(IBMX; Sigma) was added for 72 |
hours |
Chondrogenic Medium (CM) |
DMEM/F-12, SF/XF-M |
No serum |
Insulin Transferrin-Selenium+1 |
(Sigma), 50 µM L- ascorbic acid, |
55µM sodium pyruvate (Life |
Technologies), 23 µM L-proline |
(Sigma), 1% GlutaMAX and 1% a/a. |
TGF-β1 (Sigma) |