Table 1

Gene	Forward primer sequence	Reversed primer sequence
H-ACTB	5’-CTGGAACGGTGAAGGTGACA-3’	5’- AAGGGACTTCCTGTAACAATGCA-3’
H-FOXA2	5’-AGGAGGAAAACGGGAAAGAA-3’	5’-CAACAACAGCAATGGAGGAG-3’
H-CXCR4^a	5’-CACCGCATCTGGAGAACCA-3’	5’-GCCCATTTCCTCGGTCTAGTT-3’
H-SOX17	5’-AAGGGCGAGTCCCGTATC-3’	5’-TTGTAGTTGGGGTGGTCCTG-3’
H-OCT4	5’-CGAAAGAGAAAGCGAACCAG-3’	5’-AACCACACTCGGACCACATC-3’
H-GSC	5’-GAGGAGAAAGTGGAGGTCTGG-3’	5’- GCAAGAAAGTAGCATCGTCTG-3’
H-ECAD	5’-CTGCCATTCTGGGGATTCT-3’	5’- CTCTTCTCCGCCTCCTTCT-3’
H-NCAD	5’-GGGTAATCCTCCCAAATCAAA-3’	5’- CCACAAACATCAGCACAAGG-3’
H-CER1	5’-CTTCTCAGGGGGTCATCTTG-3’	5’- TCCCAAAGCAAAGGTTGTTC-3’
H-TRH	5’-CTCTTCCTCCGGGAAAACAT-3’	5’-TGGGTTACATCGACTGACCA-3’
H-MIXL1^a	5’-CTCTGACGCCGAGACTTGG-3’	5’-CCAAGTCTCGGCGTCAGAG-3’
H-SOX7	5’-CACTGCTGAACTGGTCCCTAA-3’	5’-TGGCTGGTGAGGAAGACAA-3’
H-T	5’-CTACCCCAGCCCCTATGC-3’	5’-CGTTGCTCACAGACCACAG-3’
H-PYY	5’-CAGCGGTATGGGAAAAGAGA-3’	5’-GAGTGCGTATGCAAATGACG-3’
H-FGFR1	5’-GCCAGGACCCGAACAGAG-3’	5’-CCCAGAAGAGGAGGCACTT-3’
M-Sox17	5’-CTCGGGGATGTAAAGGTGAA-3’	5’-GCTTCTCTGCCAAGGTCAAC-3’
M-Foxa2^b	5’-TGGCTGCAGACACTTCCTACT-3’	5’-CAACATCAGTACAACCCTCTGGT-3’
M-Gapdh	5’-CGGTGCTGAGTATGTCGTGGA-3’	5’- GGCAGAAGGGGCGGAGATGA-3’
M-Ecad	5’-CGCTGAGATGGACAGAGAAG-3’	5’-CAGGACCAGGAGAAGAGTGC-3’
M-Ncad	5’-CAGGTTTGGAATGGGTCTGT-3’	5’-ATGTTGGGTGAAGGTGTGCT-3’
M-Cer1	5’-AGGAGGAAGCCAAGAGGTTC-3’	5’-CATTTGCCAAAGCAAAGGTT-3’
M-Oct4	5’-CACGAGTGGAAAGCAACTCA-3’	5’-AGATGGTGGTCTGGCTGAAC-3’
M-Cxcr4	5’-ACGGCTGTAGAGCGAGTGTT-3’	5’-CCGTCATGCTCCTTAGCTTC-3’
M-Trh	5’-GCTGTGACTCCTGACCTTCC-3’	5’-CCCAAATCTCCCCTCTCTTC-3’
M-Mixl1	5’-AGTTGCTGGAGCTCGTCTTC-3’	5’-AGGGCAATGGAGGAAAACTC-3’
M-Sox7	5’-GGATGAGAGGAAACGTCTGG-3’	5’-GCTTGCCTTGTTTCTTCCTG-3’
M-T ^b	5’-AACTTTCCTCCATGTGCTGAGAC-3’	5’-TGACTTCCCAACACAAAAAGCT-3’
M-Pyy	5’-GCAGCGGTATGGAAAAAGAG-3’	5’-AACACACACAGCCCTCCAGT-3’
M-Fgfr1	5’-CTGGACATCCCCAGAGAAAA-’	5’-GAAGGCACCACAGAATCCAT-3’

H=primers for human genes, M=primers for mouse genes. Primers marked with ^a or a ^bwere designed according to D′Amour and colleagues (D′Amour et al., 2005; D′Amour et al., 2006) or Tada and colleagues (Tada et al., 2005), respectively.

Table 1: Primer sequences used for quantitative RT-PCR.