Figure 4: Purity of the enriched TRA-1-60+ ECO cells. The samples were enriched by negative selection with the superparamagnetic scFv targeting CD45, CD34, CD14, CD3, CD4, CD16, CD19, CD20, dsDNA, phosphatidylserine. That was followed by positive selection with the superparamagnetic scFv targeting TRA-1-60. This resulted in the sorts’ purities > 99% with the statistical significance p < 0.001. Labels: ECO – patients’ samples (n=6); PBMC – peripheral blood mononuclear cells (n=6); BMMC – bone marrow mononuclear cells (n=6); ESC – cultures of the human embryonic stem cells H1, H7, H9 (n=3); ECT -cultures of the human testicular embryonal carcinoma cells NT2D1 (n=1). |