Figure 14: Formation of embryoid bodies and differentiation into three main germ layers. The embryoid bodies were tested by labeling with the antibodies targeting neurofilaments protein (68kDa) as the biomarker for the forming ectoderm; muscle myosin for the mesoderm; and cytokeratins for the endoderm. Labels: the embryoid bodies from the ECO cells (A-F), embryonic stem cells (H7) (G); (negative controls not included); +: positive reaction; -: negative reaction; L: lost sample.