Figure 2: The stem cells biopsied directly from the primary tumors of the patients diagnosed with the embryonal carcinoma of the testes (in the figure 1 for the patient encoded 001) were triple-labeled with the Fvs targeting DNA (violet), TRA-1-60 (green), and SSEA-4 (red), which were tested for specificity and sensitivity on blots as shown in the figure 1. All the batches for every patient were labeled in triplicates. The images presented in the figure are representative to all the patients and the controls studied. From each batch, the images of ten randomly selected cells were acquired at the three acquisition channels without correcting the wavelength induced shift. The images were acquired with multiphoton excitation fluorescence. The HFW: 30μm.