Figure 1: The expression of germ cell-specific genes in human amniotic epithelial cells (hAECs) (A) Quantitative PCR was used to analyze germ cell specific expression in hAECs. CT values were expressed as a percentage of 18S RNA (18S=100%) and used to calculate mean normalized expression relative to 18S. Results were shown as mean and standard deviation of three experiments. (B) Immunofluorescence analysis of germ cell-specific genes in hAECs. Note that hAECs expressed OCT4 (red), NANOG (green) and CD117 (green); they did not express DAZL, STELLA or VASA. DAPI staining for nuclei. Scale bars=50μm.