Figure 9: Expression of the OCT4 gene in the embryoid bodies (EB) differentiating for over sixty days from the human induced pluripotent stem cells was studied by quantitative polymerase chain reaction after reverse transcription (qRTPCR). At that time point, in the EBs, there were detectable contractions. The expression values were calculated with the reference to GAPDH, while relative to the cultured undifferentiated embryonic stem cells. The samples were run in triplicates. The graph is representative for all the samples studied. Differentiation of these cells resulted in down regulated gene expression for the OCT4. The statistical significance was accepted at p < 0.001.