Figure 10: Cell surface display of the biomarkers SSEA-4, SSEA-3, TRA-1-60, and TRA-1-81, as the hallmark of pluripotency, was studied with the confocal fluorescence. The human primary culture fibroblast (hpcF) of this patient (encoded C003) expressed fibroblast growth factor receptor (FGFR), but none of the pluripotency receptors (blank fields of view not shown). HFW: 60 μm. However upon induction, the fibroblasts from these cultures changed their geometry, as the first sign of the successful induction, and expressed on their surfaces these pluripotent biomarkers (SSEA-4, TRA-1-60), highlighted by labeling with the htAbs. The staining of the genomic (gDNA) served as the internal reference of the nucleus. The samples were run in triplicates. The data are representative for all the samples studied. HFW: 20 μm.