Figure 3: Analysis of cell viability. Raw data from representative analysis of Annexin (Ann) V expression and 7-AAD staining in UCBMSCs treated with camptothecin, used as positive control for cell apoptosis, calphostin C and U0126, and following transduction with siEgr-3. Ann+7-AAD- and 7-AAD+ label early and late apoptotic cells, respectively. The percentage of live cells under each condition is indicated in the corresponding dot plot. Treatment with camptothecin induced a moderate amount of programmed cell death, indicative of intrinsic UCBMSC robustness. The 10-fold increase in the fluorescence emitted by calphostin C-treated cells did no effect measured levels of cell death. N=2 for each experimental condition with similar results.