Figure 8: Vimentin (green), CK19 (green), and NGN3 (red) expression during the first trimester (7.7, 8.4, 14.4) and post natal (10 wpn). At 7.7 wpc the pancreas is composed of vimentin positive cells (mesenchyme). NGN3 positive cells are lining a lumen and all the NGN3 positive cells co-express vimentin (inset). At 14.4 wpc, there are less vimentin positive cells and the NGN3 positive cells surround cell clusters (forming islets). All the NGN3 positive cells co-express vimentin and are also visible within the islet structure. NGN3 cells at 10 wpn are scattered throughout the pancreas and within the islet, these cells co-express vimentin. At 8.4 wpc the ductal tree that has formed is comprised of CK19 positive cells. NGN3 positive cells are scattered in and amongst the spaces around the ductal tree. At 14.4 wpc as the ducts are condensing and mature ducts are forming, the NGN3 positive cells are closely associated with the forming islets and are found around and within the forming islet. At 10 wpn the NGN3 positive cells are scattered throughout the section and do not appear to be associated with the mature ducts (CK19). Areas denoted by a box are shown at higher magnification (inset). Scale bars are 50 and 10 μM respectively.