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Figure 1: Schematic representation of the derivation of human pancreatic distributed stem cells. Donor human cadaveric pancreas fragments were minced and serially digested. Released cells and islets were contained in two consecutive supernatant fractions (DF1 and DF2). A third fraction (DF3) was composed largely of residual tissue fragments. Material from all three fractions was cultured under 4 different conditions. Ctrl, no SACK agent supplementation; or supplementation with one of each of the three SACK agents, Xs, Xn, and Hx. |