Figure 4: SACK-expanded HuPan DSC strains co-express pancreatic endocrine and mesenchymal markers under conditions of active proliferation. Example of ISIF micrographs for HuPan DSCs showing positive nuclear staining for the endocrine progenitor transcription factor Ngn3 (top panels) and positive cytoplasmic staining for the pancreatic lineage marker Glut2 (middle panels) and the mesenchymal cell marker vimentin (bottom panels). DAPI, nuclear DNA epifluorescence. Scale bars, 50 μm.