Figure 5: SACK-expanded HuPan DSC strains produce differentiated cells that express insulin and glucagon. A. Phase micrograph showing examples of cell clusters produced after 4 days of culture of HuPan cell strains under conditions that promote pancreatic lineage differentiation. Scale bar, 100 μm. B. Scheme for characterization of cell lineage phenotypes in differentiated cell clusters by ISIF (See Materials and Methods). C. Representative epifluorescence micrographs of HuPan cells after ISIF with specific anti-Ngn3, anti-Glut2, antiinsulin, and anti-glucagon antibodies as indicated. DAPI, nuclear DNA epifluorescence. Scale bars, 50 μm.