Figure 6: Differentiated progeny cells produced by clonal HuPan DSCs co-express insulin and glucagon. Shown are examples of epifluorescence micrographs of ISIF analyses of cryosections of differentiated cell clusters produced from a clonal HuPan DSC strain. Top row, anti-Ngn3. Second row, anti-Glut2. Third row, dual ISIF with anti-insulin and anti-glucagon antibodies. Arrowheads, indicate examples of cells expressing only insulin. Arrows indicate examples of cells producing only glucagon. Bottom row, anti-C-peptide. DAPI, nuclear DNA epifluorescence. Scale bars, 50 μm.