List of ECM related genes differentially expressed in step 1 and 2 and their fold change (FC) in comparison to MX3
S No. Gene IDs Gene Name FC (Step1) FC (Step 2)
1 1100001G20Rik RIKEN cDNA 1100001G20 gene -11.839 -13.619
2 4930486L24Rik RIKEN cDNA 4930486L24 gene   3.1914
3 5430419D17Rik RIKEN cDNA 5430419D17 gene   -3.7122
4 9030224M15Rik RIKEN cDNA 9030224M15 gene -2.8859 -3.2284
5 9030425E11Rik RIKEN cDNA 9030425E11 gene   -1.7351
6 Acp5 acid phosphatase 5, tartrate resistant   -1.6132
7 C130099A20Rik acid phosphatase-like 2   -2.7857
8 Adamts12 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 12   -1.9985
9 Adamts4 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 -1.7856  
10 Adamts7 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 7   -2.771
11 Adamts9 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9   -2.2331
12 Adipoq adiponectin, C1Q and collagen domain containing -13.224 -6.2983
13 Ager advanced glycosylation end product-specific receptor   4.7325
14 Akp2 alkaline phosphatase, liver/bone/kidney -2.2072  
15 Angpt2 angiopoietin 2   1.9482
16 Angpt4 angiopoietin 4   -1.9422
17 Angptl4 angiopoietin-like 4 -1.8673 -2.6075
18 Anxa1 annexin A1   2.0009
19 Apob apolipoprotein B   -2.5145
20 Apoc1 apolipoprotein C-I   -3.765
21 Aqp1 aquaporin 1   -2.8826
22 Bmp1 bone morphogenetic protein 1   -2.0406
23 Bmper BMP-binding endothelial regulator   -5.4121
24 Calu calumenin   -1.8206
25 Camp cathelicidin antimicrobial peptide -2.0961 -10.138
26 Ccdc3 coiled-coil domain containing 3   3.5129
27 Ccdc80 coiled-coil domain containing 80   -2.0505
28 Ccl2 chemokine (C-C motif) ligand 12   -4.9783
29 Ccl2 chemokine (C-C motif) ligand 2   -4.9783
30 Cd109 CD109 antigen   1.9406
31 Fap carboxyl ester lipase -2.4267 -9.1509
32 Cfb complement factor B   -3.6377
33 Cfd complement factor D (adipsin) -38.614  
34 Tna;Clec3b C-type lectin domain family 3, member b   -3.1921
35 Cma1 chymase 1, mast cell 2.3672  
36 Cml3 camello-like 3   -1.9007
37 Col16a1 collagen, type XVI, alpha 1   -1.7936
38 Col1a2 collagen, type I, alpha 2   -2.6495
39 Col28a1 collagen, type XXVIII, alpha 1 1.9029  
40 Col3a1 collagen, type III, alpha 1   -5.1442
41 Col5a1 collagen, type V, alpha 1   -2.138
42 Col5a2 collagen, type V, alpha 2   -3.1669
43 Col6a2 collagen, type VI, alpha 2   -2.9376
44 Col6a3 collagen, type VI, alpha 3   -3.2754
45 Col8a1 collagen, type VIII, alpha 1   -2.5865
46 Colec10 collectin sub-family member 10   -3.0225
47 Creg1 cellular repressor of E1A-stimulated genes 1   1.5715
48 Ctla2a cytotoxic T lymphocyte-associated protein 2 alpha   4.8841
49 Cxcl1;Cxcl2 chemokine (C-X-C motif) ligand 1   -2.9
50 Cxcl12 chemokine (C-X-C motif) ligand 12   -2.8228
51 Cxcl14 chemokine (C-X-C motif) ligand 14   -6.0245
52 Zmynd15;Cxcl16 chemokine (C-X-C motif) ligand 16   -4.3483
53 Cxcl2 chemokine (C-X-C motif) ligand 2   -1.6807
54 Cxcl1 chemokine (C-X-C motif) ligand 3   -2.910
55 Dcn decorin -6.1048 -12.857
56 Dkk3 dickkopf homolog 3 (Xenopus laevis) -2.007  
57 Dpt dermatopontin -2.8247 -15.829
58 Dpysl3 dihydropyrimidinase-like 3   -4.7569
59 Edn1 endothelin 1   3.0253
60 Endod1 endonuclease domain containing 1 -1.5923  
61 Enpp1 ectonucleotide pyrophosphatase/phosphodiesterase 1   -2.9094
62 Enpp2 ectonucleotide pyrophosphatase/phosphodiesterase 2 -1.8782  
63 Enpp3 ectonucleotide pyrophosphatase/phosphodiesterase 3   -3.4383
64 Epdr1 ependymin related protein 1 (zebrafish)   -2.0888
65 Erap1 endoplasmic reticulum aminopeptidase 1   -1.757
66 Esm1 endothelial cell-specific molecule 1 -1.89 -13.513
67 F13a1 coagulation factor XIII, A1 subunit -4.7944 -29.179
68 F8 coagulation factor VIII   2.3108
69 Fas Fas (TNF receptor superfamily member 6)   -2.2147
70 Fetub fetuin beta   4.0025
71 Fgf7 fibroblast growth factor 7   -1.8952
72 Fgfr2 fibroblast growth factor receptor 2   2.6829
73 Fgl1 fibrinogen-like protein 1   -3.9173
74 Fst follistatin   2.9435
75 Fxyd5 FXYD domain-containing ion transport regulator 5 -1.9048 -2.8009
76 Gbp1 guanylate binding protein 1   -2.8321
77 Gbp2 guanylate binding protein 2   -2.2818
78 Dbp group specific component   -2.0841
79 Gdf15 growth differentiation factor 15   4.8264
80 Gpc1 glypican 1   -5.5069
81 Gpi1 glucose phosphate isomerase 1   -2.0234
82 Grem1 gremlin 1   -2.1274
83 Gria3 glutamate receptor, ionotropic, AMPA3   1.6481
84 Gsn gelsolin -4.1321 3.2729
85 Gstm1;Gsta3 glutathione S-transferase, mu 1   6.1158
86 Hgf hepatocyte growth factor   -5.9014
87 Hp haptoglobin   -1.7199
88 Hsd17b11 hydroxysteroid (17-β) dehydrogenase 11   2.9423
89 Htra1 HtrA serine peptidase 1   -1.6745
90 Igf1 insulin-like growth factor 1   -1.8976
91 Igfbp4 insulin-like growth factor binding protein 4 -3.5146  
92 Il13ra2 interleukin 13 receptor, α 2   -1.7713
93 Il15 interleukin 15 -1.7454  
94 Il16 interleukin 16   -4.2025
95 Il1rl1 interleukin 1 receptor-like 1   -1.9204
96 Il33 interleukin 33   -2.951
97 Il4ra interleukin 4 receptor, α   -2.1844
98 Hgf interleukin 6   -5.9014
99 Il7 interleukin 7   -4.6948
100 Insl3 insulin-like 3 -2.3022 -2.2062
101 Itgbl1 integrin, beta-like 1 -2.591 -3.1682
102 Kng1 kininogen 1   -2.745
103 Lbp lipopolysaccharide binding protein   3.2792
104 Lefty1 left right determination factor 1   -1.6338
105 Lepre1 leprecan 1   -1.6405
106 Lrg1 leucine-rich alpha-2-glycoprotein 1   -4.6728
107 Mcpt8 mast cell protease 8 2.9043  
108 Hgf met proto-oncogene   -5.9014
109 Mfap5 microfibrillar associated protein 5   -2.189
110 Mgp matrix Gla protein (glypican 3)   4.2015
111 Mmp13 matrix metallopeptidase 13 -1.7855 -11.849
112 Mmp3 matrix metallopeptidase 3 -3.3833 -17.747
113 Mmp9 matrix metallopeptidase 9   -5.0587
114 Msln mesothelin 1.6651  
115 Nid2 nidogen 2   -2.6617
116 Olfml3 olfactomedin-like 3   -6.1867
117 Orm1;Orm2 orosomucoid 1 -5.2841  
118 Pla1a phospholipase A1 member A   1.7027
119 Prelp proline arginine-rich end leucine-rich repeat   3.2311
120 Prg4 proteoglycan 4   3.9319
121 Prl2c2 prolactin family 2, subfamily c, member 2 3.1404 3.4205
122 Prl2c4 prolactin family 2, subfamily c, member 3 2.7615 3.0803
123 Prl2c4 prolactin family 2, subfamily c, member 4 2.7615 3.0803
124 Prrg2 proline-rich Gla (G-carboxyglutamic acid) polypeptide 2   -1.6326
125 Prss23 protease, serine, 23   -2.4939
126 Ptgds prostaglandin D2 synthase (brain)   5.6525
127 Ptn pleiotrophin 1.7609 -2.0464
128 Tns1 PYD and CARD domain containing   6.0674
129 Lbp ribosomal protein SA   3.2792
130 S100a1 S100 calcium binding protein A13   2.0034
131 S100a8 S100 calcium binding protein A8 (calgranulin A) -3.5527 -3.6393
132 Saa1 serum amyloid A 1   -8.4299
133 Saa3;Saa1 serum amyloid A 3   -35.708
134 Scgn secretagogin, EF-hand calcium binding protein   2.0983
135 Selp selectin, platelet -2.0813 -2.1803
136 Sema3a sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A   -2.0095
137 Sema3c sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C   -2.9553
138 Sema3e sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E   2.9479
139 Sepp1;Selp selenoprotein P, plasma, 1 -2.0813 3.3669
140 Serpina3f serine (or cysteine) peptidase inhibitor, clade A, member 3F   -1.7811
141 Serpina3g serine (or cysteine) peptidase inhibitor, clade A, member 3G   -4.1345
142 Serpinb1a serine (or cysteine) peptidase inhibitor, clade B, member 1a   -36.861
143 Serpine2 serine (or cysteine) peptidase inhibitor, clade E, member 2   -1.9456
144 Serping1 serine (or cysteine) peptidase inhibitor, clade G, member 1   -1.786
145 Sfrp1 secreted frizzled-related protein 1   -1.9697
146 Sfrp2 secreted frizzled-related protein 2   -3.2157
147 Slc1a3 solute carrier family 1 (glial high affinity glutamate transporter), member 3   -1.7185
148 Slpi secretory leukocyte peptidase inhibitor   -12.206
1549 Sparc secreted acidic cysteine rich glycoprotein -1.6497 -2.3295
150 St6gal1 beta galactoside alpha 2,6 sialyltransferase 1   1.6493
151 Stc1 stanniocalcin 1   -13.677
152 Sulf1 sulfatase 1   -1.8901
153 Tfrc transferrin receptor   -2.1891
154 Tgfbi transforming growth factor, beta induced -2.1783 -3.8837
155 Thbd thrombomodulin   -3.1583
156 Timp1 tissue inhibitor of metalloproteinase1   -3.0244
157 Timp4 tissue inhibitor of metalloproteinase4   5.6433
158 Tinagl tubulointerstitial nephritis antigen-like 1   3.2907
159 Tnfrsf11b tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) -3.0351 -2.5493
160 Tnfsf13b tumor necrosis factor (ligand) superfamily, member 13b   -1.6894
161 Tnxb tenascin XB   -2.1409
162 Tpsab1 tryptase alpha/beta 1 -5.5514 -13.068
163 Tpsab1 tryptase beta 2 -5.5514 -13.068
164 Trem2 triggering receptor expressed on myeloid cells 2   -2.1177
165 Hp transferrin   -1.7199
166 Vcam1 vascular cell adhesion molecule 1   1.6674
167 Vcan versican   -2.7587
168 Vegfa vascular endothelial growth factor A -2.3108 -3.7255
169 Vnn3 vanin 3 -1.9471 -2.049
170 Vtn vitronectin   -3.3449
171 Wfdc12 WAP four-disulfide core domain 12 -3.2524 -4.1203
172 Wnt4 wingless-related MMTV integration site 4   1.9837
Table 1: List of ECM related genes differentially expressed in step 1 (increase in oxygen) and 2 (increase in oxygen and addition of osteogenic media) and their fold change in comparison to MX3. The numbers with minus sign (-) indicate their down regulation in that step.