Figure 4: Comparison of mean population doubling time (PDT) for equine AD-MSCs cultured in different medium combinations and seeding densities at (A) passage 1, (B) passage 2 and (C) passage 3. The results are average ± S.E.M. of 3 culture replicates for each passage. * indicates P<0.05, ** indicates P<0.01 compared to the 1000 seeding density.