Figure 3: Differentiation of human TBK1-associated iPSCs into retinal ganglion cell-like neurons. (A) Schematic of methods used to produce retinal ganglion cell-like neurons. (B) rt-PCR analysis of RNA isolated from TBK1-iPSC or WT-iPSC derived retinal ganglion cell-like neurons shows expression of markers expressed by RGCs. (C and D) Immunocytochemical analysis of TBK1-iPSC derived retinal ganglion cell-like neurons targeted against the neural/retinal ganglion cell markers NF200 (C) and ATOH7 (D). (E) Schematic diagram illustrating the paradigm utilized to isolate/purify TBK1-iPSC derived retinal ganglion cell like neurons from a heterogeneous culture of differentiated cells. (F) Microscopic/morphological analysis of TBK1-associated iPSC-derived retinal ganglion cell-like neurons postmagnetic bead isolation. Scale bar=200 microns for panel C and D, and 400 microns for panel F.