Figure 1: Isolation and cytogenetic characterization of isolated prostate cells from BPH patient. (A) Schematic diagram of prostatic cells isolation from TURP tissue. Cells were obtained after 1 hrs, incubation with collagenase type-I enzyme in DMEM medium without FBS at 37°C. (B and C) Morphological view of isolated cells from mixed population using sterile filter paper discs soaked in trypsin and cultured in DMEM medium with FBS at 37°C under 5% Co2 .(D) karyotype analysis of isolated prostate cells. Cells showed aneuploidy with 4 to 5 marker chromosomes and translocation of 6th chromosome to 1st.