Figure 6: Isolated prostate cells can differentiate to endodermal cell lineages. (A) Without and (B) with islet differentiation reagents (serum free RPMI 1640 medium with 10 ng/l activin-A and 10 mg/l insulin) for 10 days. (A) Control. (B) Islet like cell cluster. (C) DTZ staining. (D) islet-like cell cluster were detected by immunofluorescence staining using C-peptide and glucagon antibodies. Nuclei were stained with DAPI.