Gene Primer sequence Annealing Temp. Product size
Oct 3/4 (NM_002701) Forward-5’AGCTGGAGAAGGAGAAGCTGG-3’ Reverse-5’-TCGGACCACATCCTTCTCGAG-3’ 63.5°C 458 bp
Sox-2 (NM003106) Forward-5’-CACCTACAGCATGTCCTACTC-3’ Reverse-5’-CATGCTGTTTCTTACTCTCCTC-3’ 60°C 384 bp
Nanog (NM_024865) Forward-5’GCAAACAACCCACTTCTGC-3’ Reverse- 5’AGGCCTTCTGCGTCACAC-3’ 55.5°C 287 bp
Table 1: Primers Sequence and annealing temperature.