| Primer Sequence (5’-3’) |
Forward |
Reverse |
| hPOU5F1 |
ATTTCACCAGGCCCCCGGCTT |
CTTTGATGTCCTGGGACTCCTCCG |
| hC-MYC |
GCAGCGACTCTGAGGAGGAACAA |
TTTTCCTTACGCACAAGAGTTCCGT |
| hLIN28 |
TCAGCCGACGACCATGGGCT |
CCATGTGCAGCTTACTCTGGTGCAC |
| hNANOG |
TGCTGGACTGAGCTGGTTGCC |
TGGAGGAAGGAAGAGGAGAGACAGT |
| hKLF4 |
GGCTGATGGGCAAGTTCG |
CTGATCGGGCAGGAAGGAT |
| hSOX2 |
CCCCTGTGGTTACCTCTTCCTCC |
TGCCGTTAATGGCCGTGCC |
| hGAPDH |
GAGTCAACGGATTTGGTCGT |
TTGATTTTGGAGGGATCTCA |
| pPOU5F1 |
ACAAGGAGAAGCTGGAGCCG |
CGCGGACCACATCCTTCTCT |
| pSOX2 |
CACCTACAGCATGTCCTACTCG |
GGTTTTCTCCATGCTGTTTCTT |
| pNANOG |
TCTGTGTCAGTTTGAGGGACAGG |
AACAAGTAAAGCCTCCCTATCCCA |
| pLIN28 |
CAGAGTAAGCTGCACATGGAGG |
GTAGGCTGGCTTTCCCTTG |
| pGAPDH |
CTCAACGACCACTTCGTCAA |
TCTGGGATGGAAACTGGAAG |
|
