Source/species Differentiation protocol Hepatocyte specific markers/functions Reference
r-MSC FGF-4+HGF+ITS+Dexa AFP, HNF3, HNF1,CK18,ALB [35]
r-MSC MSC+HGF+CNP AFP, CK18,CK19 [36]
r-MSC HGF+EGF+ Dexa ck19,  AFP, DPP IViNOS [32]
h-MSC MACS sorted CD105 + ATMSCs/ HGF+FGF1+ FGF4 /OSM+Dexa ALB, AFP, TTR, HNF-4,TDO2, CK18, TTR, ammonia detoxification, PAS staining, LDL-uptake [34]
h-MSC HGF+bFGF+ nicotinamide + OSM + Dexa CK-18, CK-19, ALB,TTR, CYP2E1, EBPβ [16]
h-MSC EGF+FGF2 / HGF + FGF2 + nicotinamide / OSM + Dexa AFP,ALB, CK18, TAT,TDO2, G6P, HNF-4, urea synthesis, CYP activity. LDL-uptake, PASstaining [37]
h-MSC HGF+OSM+Dexa+ nicotinamide AFP, CK18,ALB, TDO2,TAT,AAT, HNF-4, urea synthesis, PAS staining, CYP activity [38]
h-MSC OSM+TSA+DMSO CK18, α-FP,α-SMA [39]
Table 2: Differentiation protocol of MSCs towards Hepatocyte-Like Cells.