Figure 3: Representative Bioanalyzer electropherograms (fluorescence [ordinate] vs. time [abscissa]) with respective gels are shown for RNA extracted from 1) E13.5 intermediate (“II”, RIN: 8.9, RNA concentration: 651 pg/μl, area harvested: appr. 1021000 μm2) and outer interzone (“OI”, RIN: 9.1, RNA concentration: 952 pg/μl, area harvested: appr. 940000 μm2) and epiphyseal cartilage (“EC”, RIN: 8.4, RNA concentration: 1253 pg/μl, area harvested: appr. 930000 μm2) (top) 2) E15.5 intermediate (RIN: 9.5, RNA concentration: 489 pg/μl, area harvested: appr. 1560000 μm2) and outer interzone (RIN: 8.9, RNA concentration: 919 pg/μl, area harvested: appr. 1430000 μm2) and epiphyseal cartilage (RIN: 7.8, RNA concentration: 566 pg/μl, area harvested: appr. 3470000 μm2) (middle) 3) A fresh unstained limb section (RIN: 10, RNA concentration: 30310 pg/μl) and stained limb section after 3 hours under the microscope (RIN: 9.4, RNA concentration: 40397 pg/μl) of an E14.5 (bottom). Positions of 18S and 28S ribosomal RNA are indicated.