age group tissue group litter ID embryo ID tissue area harvested [μm2] RIN RNA harvested [ng] cRNA [ng] dyeincorp. [fmol/ng] 13.5 days gestation II 13.5-1 13.5-1-1 455829 9 4.34 950.94 12.49 13.5-1-4 900000 8.9 4.91 13.5-2 13.5-2-1 216205 1.5 1.66 570.00 17.13 13.5-2-4 479962 8.2 2.80 13.5-4 13.5-4-1 1738221 8.6 19.67 1952.10 14.80 13.5-4-2 1021397 8.9 7.81 OI 13.5-1 13.5-1-1 317121 na 2.16 1131.03 13.37 13.5-1-4 720000 8.2 6.10 13.5-2 13.5-2-1 236742 1.6 2.15 611.55 13.69 13.5-2-4 306876 8.9 5.32 13.5-4 13.5-4-1 1032708 8.6 10.98 1593.00 12.54 13.5-4-2 938646 9.1 11.42 EC 13.5-1 13.5-1-1 306100 7.4 3.44 1139.67 13.03 13.5-1-4 840000 7.5 10.08 13.5-2 13.5-2-1 590909 4.2 4.88 731.70 12.55 13.5-2-4 329599 7.9 8.88 13.5-4 13.5-4-1 712753 7.3 10.96 1296.27 13.33 13.5-4-2 926888 8.4 15.04 15.5 days gestation II 15.5-1 15.5-1-1 917089 9.3 1.93 660.00 14.00 15.5-2 15.5-2-6 1560000 9.5 5.87 1636.74 13.53 15.5-3 15.5-3-2 2484543 6.1 7.48 3630.00 15.00 OI 15.5-1 15.5-1-1 505786 6.2 1.64 490.00 13.00 15.5-2 15.5-2-6 1430000 8.9 11.03 1551.96 13.57 15.5-3 15.5-3-2 1354996 6.5 5.72 910.00 13.00 EC 15.5-1 15.5-1-1 1662168 6.5 5.21 730.00 12.00 15.5-2 15.5-2-6 3470000 7.8 6.79 1356.21 12.14 15.5-3 15.5-3-2 4206940 6.7 23.51 1710.00 13.00 List of tissue area harvested and the amount of RNA extracted from each embryo and each tissue type (intermediate interzone (II), outer interzone (OI) and epiphyseal cartilage (EC)). The RIN value of the RNA, the resulting cRNA and the dye incorporation after T7 amplification are also indicated

Table 1: Tissue area harvested and amount of RNA extracted per embryo and tissue type.