| |
13.50 |
15.50 |
| II |
OI |
EC |
all zones |
II |
OI |
EC |
all zones |
| Mean |
1.34 |
0.95 |
0.72 |
1.01 |
3.58 |
2.25 |
3.36 |
3.06 |
| Std. Dev. |
0.30 |
0.27 |
0.12 |
0.35 |
1.07 |
0.90 |
1.67 |
1.25 |
| Min |
1.00 |
0.73 |
0.63 |
|
2.66 |
1.30 |
1.79 |
|
| Max |
1.56 |
1.26 |
0.85 |
|
4.75 |
3.08 |
5.11 |
|
The calculated harvest surface area [mm2] required to obtain 10 ng total RNA
from 10 μm thick sections depending on age (13.5 and 15.5 days of gestation)
and tissue type (intermediate interzone (II), outer interzone (OI) and epiphyseal
cartilage (EC)). While RNA yield is not significantly influenced by tissue type,
70.01% of RNA yield variation is attributable to embryonic age. |
