13.50 15.50
II OI EC all zones II OI EC all zones
Mean 1.34 0.95 0.72 1.01 3.58 2.25 3.36 3.06
Std. Dev. 0.30 0.27 0.12 0.35 1.07 0.90 1.67 1.25
Min 1.00 0.73 0.63   2.66 1.30 1.79  
Max 1.56 1.26 0.85   4.75 3.08 5.11  
The calculated harvest surface area [mm2] required to obtain 10 ng total RNA from 10 μm thick sections depending on age (13.5 and 15.5 days of gestation) and tissue type (intermediate interzone (II), outer interzone (OI) and epiphyseal cartilage (EC)). While RNA yield is not significantly influenced by tissue type, 70.01% of RNA yield variation is attributable to embryonic age.
Table 2: Harvest surface area required to obtain 10 ng total RNA.