| Strengths |
Weaknesses |
| Lack of immunogenicity |
Heavy surgery :puncture of the bone marrow |
Regulation of crucial biological functions:
• Proliferation Angiogenesis
• Differentiation Epithelization
• Inflammation
|
Low amount of MSC in bone-marrow
(1/10000 nucleated cells) |
Expansion in vitro obtained in not complex culture medium:
• Basic fibroblast growth factor-enriched media.
• Serum-enriched/serum-free media
• Cytokine-free media
|
With increasing passage number:
• Decrease of proliferation rate
• Loss of their typical morphology
|
Good expansion yield in culture with retain of:
• Growth
• Multilineage potential.
Stable morphology (fibroblast) up to 20-25 passages
No chromosomal abnormalities for MSC expanded in vitro |
The discrepancy between pro or anti-tumoral effect of MSC related to:
• Different tissue sources
• Culture conditions
• Individual donor variability
• Injection timing of MSC
|
| Isolation efficiency of BM-MSC : 100% |
Decrease of differentiation potential with age of donor |
