Figure 2: Schematic representation of the different steps for 3D reconstruction of bladder equivalent from ASCs.
A: Adipose derived stem cells (ASCs) are cultivated with Ascorbic acid for 21 days.
B: Superimposition of cell sheets follows
C: 4 days are allowed for fusion of the cell sheets to form the of the reconstruct.
D: Urothelial cells are seeded on top of the reconstruct
E: The reconstruct is cultured submerged for 7 days to give time for urothelial cells to proliferate.
F: The reconstruct is placed at the air-liquid interface for urothelial differentiation.