Figure 6: Expression patterns of TCR Vβ genes in CTLs co-cultured with autologous DCs expressing recombinant B*44/ 156 variants. RNA was extracted from CTLs co-cultured with autologous DCs expressing recombinant B*44/ 156 variants, following which cDNA was synthesized and specific TCR Vβ genes were amplified by nested PCR. We found differential patterns in the expression of Vβ genes among B*44:02 specific CTLs cultured in the presence of autologous DCs expressing B*44:02 (6a), B*44:03 (6b) and B*44:35 (6c). M, 10bp ladder; -ve, negative control; Vβ1-24 – TCR Vβ subfamilies.