Figure 1: Overview of SVF isolation from fat tissues. In the following experiments, the fat tissues were all excised from abdominal subcutaneous tissues. (A) 3 g of the excised fat tissues were placed in the culture dishes and cut into small fragments either (B) by surgical scissors (conventional method) or (C) by disposable disperser tube method (our newly developed technique). (D) Samples were digested with collagenase in hybridization oven and (E-F) SVF pellets were generated by centrifugation.