Figure 5: The osteogenic differentiation of the ADSCs. After incubation in the different culture media for 6 days (n=3), the ADSCs were then exposed to the differentiation medium for 14 days and examined for their osteogenic differentiation. Photomicrographs show alkaline phosphatase (ALP) activity (A) and von Kassa staining (B) of the ADSCs. Bar=500 µm. Both ALP and von Kassa staining demonstrate osteo-differentiation of the ADSCs. (C) The expression of osteogenesis-associated genes in the ADSCs quantified by quantitative PCR analyses, using β-actin as the internal control. Each bar represents mean cell density ± SD. *p<0.05. **p<0.01. ***p<0.005.